Effect of LINC00662 on the malignant biological behavior of ovarian cancer cells by regulating the miR-424-5p/YAP1 axis
Objective To investigate the effect of long non-coding RNA LINC00662(LINC00662)on the malignant biological behavior of ovarian cancer(OC)cells by regulating the microRNA-424-5p(miR-424-5p)/Yes associated protein 1(YAP1)axis.Methods qRT-PCR method was applied to detect the mRNA levels of LINC00662,miR-424-5p,and YAP1 in ovarian cancer tissues and cells.Dual luciferase assay was applied to detect the targeting relationship between LINC00662 and miR-424-5p,and between YAP1 and miR-424-5p.SKOV3 cells were separated into Control group,sh-NC,sh-LINC00662,sh-LINC00662+anti NC,sh-LINC00662+anti miR-424-5p.The proliferation,apoptosis,migration,invasion and protein expression of SKOV3 cells were detected.The effect of LINC00662 on the growth of ovarian xenografts was detected.Results LINC00662 and YAP1 expression were upregulated in ovarian tissue and cells,while miR-424-5p expression was downregulated(P＜0.05).Bioinformatics and dual luciferase experiments reveled that LINC00662 had a targeted binding site with miR-424-5p,and YAP1 had a targeted binding site miR-424-5p.Compared with the sh-NC group,the positive rate of Edu,the rate of scratch healing,the number of invasive cells,the expression levels of LINC00662,YAP1 and Ki67 in the sh-LINC00662 group were decreased,the tumor volume and weight were decreased,and the apoptosis rate,the expression levels of miR-424-5p and caspase-3 were increased(P＜0.05).Low expression of miR-424-5p attenuated the inhibitory effect of silencing LINC00662 on the malignant biological behavior of SKOV3 cells.Conclusion LINC00662 is obviously upregu-lated in OC cells and tissues.Silencing the expression of LINC00662 may inhibit OC cell proliferation,migration,invasion,and promote cell apoptosis by regulating the miR-424-5p/YAP1 axis.
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