The effect of miR-214-3p expression pattern on iron death in breast cancer and its association with clinicopathological features
Objective To investigate the effect of miR-214-3p expression pattern on iron death in breast cancer and its correlation with clinicopathological features.Methods A total of 144 breast cancer patients admitted to The Affiliated Lianyungang Oriental Hospital of Kangda College of Nanjing Medical University from February 2020 to March 2023 were selected as the study objects to analyze the relationship between miR-214-3p expression level and clinical characteristics.Hierarchical regression model was used to analyze the relationship between different clinical features and miR-214-3p expres-sion.The patients were also divided into death group(n=50)and survival group(n=94)according to the prognosis.The gen-eral data and iron-death related indicators of the two patients were compared,and the relationship between miR-214-3p ex-pression and iron-death related proteins was analyzed by Logistic regression analysis and correction model.The correlation between miR-214-3p expression and death and the dose-response relationship were analyzed by unconditioned Logistic re-gression and restricted cubic spline model.Subgroup analysis of the correlation between miR-214-3p expression level and prognosis was performed according to the median tangential point.Kaplan-Meier survival curve was used to verify the effect of miR-214-3p expression on patient survival.Results Hierarchical regression analysis showed that histological grade,TNM stage,tumor size,molecular type,tumor differentiation degree and distant metastasis had significant effects on the expression of miR-214-3p(P＜0.05).There were significant differences in ferrous ion(Fe2+),malondialdehyde(MDA)and glutathione(GSH)among breast cancer patients with different prognosis(P＜0.05).Logistic regression analysis showed that after adjusting for confounding factors,transferrin,transferrin receptor 1(TfR1),ferritin transporter(FPN)and ferritin heavy chain 1(FTH1)were closely related to the expression of miR-214-3p(P＜0.05).The results of the restricted cubic spline model showed that the expression level of miR-214-3p was negatively correlated with the intensity of the association with death(P＜0.05),and there was a nonlinear dose-response relationship(P＜0.05).Subgroup analysis showed that the relationship between miR-214-3p expression and prognosis was stable,and there was no interaction between subgroups(Pinteraction＞0.05).The survival rates of patients with low expression of miR-214-3p and patients with high expression of miR-214-3p were statistically different(P＜0.05).Conclusion The low expression of miR-214-3p is closely related to the progression of breast cancer,and affects the iron death of breast cancer cells by regulating the protein pathway associated with iron death.
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