The impact of early enteral nutrition in combination with microecological preparation on the intestinal flora of children with acute pancreatitis was investigated using metagenomics analysis
Objective The impact of early enteral nutrition in combination with microecological preparation on the intestinal flora of children with acute pancreatitis was investigated using metagenomics.Methods A total of 156 pediatric patients diagnosed with acute pancreatitis and admitted to Children's Hospital of Soochow University between January 2021 and December 2023 were enrolled in this study.They were randomly assigned to either the combination group(n=78)or the control group(n=78)using a random number method.Both groups received standard treatment,while the control group also received early enteral nutrition support,and the combination group was additionally administered microecological prepara-tions.Clinical data from both groups were compared,intestinal stool samples were collected for metagenomic detection,and biological information analysis was conducted to compare changes in the intestinal flora of patients in both groups.Results After the initiation of treatment,there were no significant differences in clinical data,α diversity and β diversity,in-testinal flora number,species composition and relative abundance between the two groups(P＞0.05).Following treatment,statistically significant differences were observed in WBC,ALB,LIP,AMY,CRP,PCT,α diversity,β diversity,intestinal flora number,species composition and relative abundance between the two groups(P＜0.05).The Lefse method was employed to analyze the dominant intestinal bacteria of both groups.The findings revealed that prior to treatment Bacteroides and Es-cherichia were the dominant bacteria in both the control group and combination group.Post-treatment analysis showed that Bacteroides,Lactobacillus,and Prevotella became dominant species in the control group while Bacteroides,Lactobacillus,Prevotella,and Bifidobacterium emerged as dominant genera in the combination group.KEGG functional enrichment analysis indicated no significant differences in gene expression across all functional categories between the two groups before treat-ment;however post-treatment differential genes between control group and combination group were significantly enriched in key pathways including glucose metabolism amino acid metabolism carbohydrate metabolism lipid metabolism and signal transduction.Conclusion The combination of early enteral nutrition and microecological preparations has a remarkable effect on improving the intestinal flora of children with acute pancreatitis.
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