Effect of prenatal stress on skin development and TLR4 expression in offspring
Objective To study the effects of prenatal stress on skin development and Toll-like receptor 4 expression in offspring.Methods The prenatal stress pregnant rat model was replicated using the chronic unpredictable mild stress(CUMS).Pregnant rats were divided into Control and Model groups,and the offspring rats were divided into Control-0 and Model-0 groups(on the day of birth),and Control-60 and Model-60 groups(60 days after birth),ELISA kits were used to detect serum glucocorticoid(GC)and interleukin-6(IL-6)content in rats,H-E staining was used to observe the pathological changes of the skin in the offspring on the 0th and 60th day,the skin thickness and the number of hair follicles were measured,Masson staining was used to observe the collagen content and muscle fibers content,and immunofluorescence were used to detect the expression of TLR4 in skin tissues.Results Compared with the Control group,the serum GC and IL-6 levels of female rats in the Model group were significantly increased(P＜0.01).Compared with the Control-0 group,the serum GC and IL-6 levels of offspring in the Model-0 group were significantly increased(P＜0.05),the thickness of the whole skin,the num-ber of hair follicles,the collagen fiber content and muscle fiber content were significantly decreased(P＜0.01),the skin TLR4 protein expression was significantly increased(P＜0.01).Compared with Control-60 group,the thickness of the whole skin,the number of hair follicles,and muscle fiber content were significantly decreased(P＜0.01),the skin TLR4 protein expression was significantly increased(P＜0.01)in the Model-60 group.Conclusion Prenatal stress inhibited offspring skin growth and de-velopment,and promoted offspring skin TLR4 protein expression.
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