Objective To investigate the effect of acacetin on the malignant progression of cervical cancer cells by regulating the immune response mediated by the stimulator of interferon gene(STING)/TANK binding kinase 1(TBK1)/interferon regulatory factor 3(IRF3)pathway.Methods HeLa cells were separated into blank group,low,medium,and high concentration Aca groups(Aca-L group,Aca-M group,Aca-H group),STING inhibitor(H-151)group,and Aca-H+H-151 group.EdU staining and CCK-8,Transwell and scratch assay were used to detect cell proliferation,invasion and migration,respectively.Western blot was applied to detect STING,p-TBK1,and p-IRF3 proteins.The HeLa cells and CD8+T cells in the above groups were co cultured and were sequentially named HeLa+CD8+T group,CD8+T+Aca-L group,CD8+T+Aca-M group,CD8+T+Aca-H group,CD8+T+H-151 group,and CD8+T+Aca-H+H-151 group.Detect levels of per-forin,interferon-γ,and granzyme B in the supernatant of co culture systems and the effect of CD8+T cells on the cytotoxicity of HeLa cells.Results Compared with the blank group,the proliferation,invasion and migration of HeLa cells decreased,the STING,p-TBK1,and p-IRF3 proteins were upregulated in Aca-L,Aca-M and Aca-H groups(P＜0.05),the change trend of corresponding indicators in the H-151 group was opposite to that in the Aca-H group(P＜0.05).Compared with the HeLa+CD8+T group,the levels of perforin,interferon-γ,and Granzyme-B secreted by CD8+T cells were increased,the cyto-toxicity of CD8+T cells against HeLa cells was enhanced in the CD8+T+Aca-L group,CD8+T+Aca-M group,and CD8+T+Aca-H group(P＜0.05),the change trend of corresponding indicators in the CD8+T+H-151 group was opposite to that in the CD8+T+Aca-H group(P＜0.05).H-151 reversed the effects of high Aca on the malignant biological behavior of HeLa cells and the immune function of CD8+T cells.Conclusion Aca may inhibit the malignant biological behavior of HeLa cells and enhance the immune function of CD8+T cells by activating the STING/TBK1/IRF3 pathway.
NETL
NSTL
万方数据
