circATG7 regulates proliferation,apoptosis,and angiogenesis of cervical cancer cells by targeting miR-628-5p
Objective To investigate the regulatory effect of autophagy related protein 7 circular RNA(circATG7)on proliferation,apoptosis,and angiogenesis of cervical cancer cells by targeting miR-628-5p.Methods Real-time quantitative PCR(qPCR)was applied to detect the expression of circATG7 and miR-628-5p in cervical cancer cell lines.HeLa cells were cultured in vitro and separated into control group,si-cicATG7 group,miR-628-5p mimics group,si-NC+NC miR-628-5p group,and si-cicATG7+miR-628-5p inhibitor group.The expression of circATG7 and miR-628-5p was detected by qPCR.Edu stain-ing and flow cytometry were applied to detect the proliferation and apoptosis.Cell angiogenesis was detected by tube forma-tion and three-dimensional culture in vitro.Immunoblotting experiments were applied to detect the expression of proteins related to angiogenesis,proliferation,and apoptosis.Dual luciferase reporter assay was applied to verify the targeted regula-tory effect of circATG7 on miR-628-5p.Results The expression of circATG7 was increased and miR-628-5p expression was decreased in cervical cancer cells(P＜0.05).Compared with the control group,the cell proliferation rate,tubular length,num-ber of vascular mimicry(VM)lumen formation,and the expression of VEGF,PCNA,and Bcl-2 protein in the si-circATG7 group and miR-628-5p mimics group were decreased,the expression of miR-628-5p,apoptosis rate,and expression of Bax protein were increased(P＜0.05).Compared with si-circATG7+miR-628-5p inhibitor group,the proliferation rate,tubular length,number of VM lumen formation,and the expression of VEGF,PCNA,and Bcl-2 protein expression of cells in the si-circATG7+miR-628-5p inhibitor group were increased,the expression of miR-628-5p,apoptosis rate,and expression of Bax protein were reduced(P＜0.05).circATG7 was able to target and downregulate miR-628-5p expression in HeLa cells.Conclusion Knocking down circATG7 can inhibit the proliferation and angiogenesis of cervical cancer cells and promote their apoptosis by upregu-lating miR-628-5p.
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