Objective To investigate the effect of the negative feedback loop of S-phase kinase associated protein 2(SKP2)/AXIN/p53 protein(P53)on radiotherapy-induced apoptosis of cervical cancer cells.Methods The model of cervical cancer radiotherapy resistance cell line ME180-RR was constructed.The mRNA and protein levels of SKP2,AXIN,and P53 were detected in cervical cancer cells induced by radiotherapy.The interaction between SKP2 and AXIN,P53 and SKP2 was detected.ME180-RR cells were divided into Control group,sh-NC group,sh-SKP2 group,pc-NC group,and pc-P53 group.The proliferation,apoptosis,and Ki67 and B lymphoblastoma-2-associated X protein(Bax)protein expression of cervical cancer cells were detected.Results The levels of SKP2 mRNA and protein in cervical cancer radiotherapy resistant cells were higher than those in cervical sensitive cells,while the mRNA and protein levels ofP53 and AXIN were lower than those in cervical sensitive cells(P＜0.05).Compared with the sh-NC group,the positive rate of EdU was decreased,the apoptosis rate was increased,the expression levels of P53,AXIN,and Bax were increased,while the expression levels of SKP2 and Ki67 were down-regulated in the sh-SKP2 group(P＜0.05).SKP2 interacts with AXIN.Compared with the pc-NC group,EdU posi-tive rate was decreased,apoptosis rate was increased,expression of Ki67 was down-regulated,expression of SKP2,P53 and Bax was up-regulated in pc-P53 group(P＜0.05).P53 has a targeting effect on SKP2.Conclusion The expression of SKP2 increases in cervical cancer cells after radiotherapy,and SKP2 forms a negative feedback loop with AXIN and P53.Inhibiting SKP2 expression can inhibit the radiotherapy resistance of cervical cancer and promote cell apoptosis.
cervical cancerS-phase kinase associated protein 2radiation therapyapoptosis
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