High-throughput sequencing identifies HBG2 and HBZ as key genes for insulin-resistant recurrent miscarriage
Objective A sequencing library of chorionic villus tissue from pregnant women with recurrent spontane-ous abortions combined with insulin resistance was constructed for differential expression profiling and functional enrichment analysis,and key genes were identified and screened to elucidate the mechanism of recurrent spontaneous abortions combined with insulin resistance.Methods Chorionic tissue samples from 4 pregnant women with recurrent spontaneous abortions combined with insulin resistance(experimental group)and 4 pregnant women with induced abortion(control group)were collected in the family planning clinic of Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University from December 2023 to May 2024.High-throughput sequencing technology was applied to construct sequencing libraries,screen differentially ex-pressed genes,perform gene ontology,gene set enrichment analysis,genome encyclopedia analysis and protein interactions network,and qRT-PCR for experimental validation after screening key genes by Cytoscape.Results A total of 64 differentially expressed genes were screened by high-throughput sequencing,including 8 up-regulated genes and 56 down-regulated genes.GO enrichment analysis showed that the bioprocesses were enriched in protein hydrolysis,the cellular components were lo-cated in the plasma membrane,and the molecular functions acted in binding to heme.GSEA was enriched in the HALLMARK_E2F_TARGETS gene set,and the up-regulated gene KEGG was enriched in the Chronic Hyperglycemia Im-pairment neuronal function pathway,and down-regulated gene KEGG is enriched in oxygen transport pathway.HBG2 and HBZ were identified as key genes by Cytoscape MCODE,and qRT-PCR experiments verified that they were significantly down-regulated in the experimental group.Conclusion HBG2 and HBZ are potential bioinformatics markers for recurrent spontaneous abortions combined with insulin resistance,while the oxygen transport pathway may play a key role in the de-velopment.
recurrent spontaneous abortions combined with insulin resistancehigh-throughput sequencingchori-onic tissue