The ameliorating effect of lidocaine induced labor analgesia on hypoxia in neonatal rats by regulating the MAPK signaling pathway
Objective To investigate the ameliorating effect of lidocaine induced labor analgesia on hypoxia in neo-natal rats by regulating mitogen-activated protein kinase(MAPK)signaling pathway.Methods Ten pregnant rats were ran-domly grouped into normal delivery group,delivery asphyxia group,lidocaine group,anisemycin group,lidocaine+anisemycin group,with 2 rats in each group.Except for the normal delivery group,the rats in the other groups were induced asphyxia on the 21st day of pregnancy,and the pregnant rats were administered when the contractions began and the pregnant rats were about to give birth.A total of 12,13,12,14,and 12 newborn rats were delivered in the normal delivery group,the delivery asphyxia group,the lidocaine group,the animomycin group,and the lidocaine+anitomycin group,respectively,so 12 newborn rats were included in each group for follow-up studies.Morris water maze test was used to detect the escape latency,the time spent exploring space,and the number of times crossing platforms.H-E staining was used to detect the brain histopathology in the CA1 region of the hippocampus of neonatal rats.Transmission electron microscopy was used to observe the synaptic mor-phology of neonatal rats.Kits was used to detect the levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in the CA1 region of hippocampus.TUNEL staining was used to detect neuronal apoptosis in the CA1 region of the hippocampus.Western blot was used to detect Bim,p53,p-p38 MAPK and p-ERK proteins in the CA1 of hippocampus.Results Compared with the delivery asphyxia group,the escape latency and time spent exploring space of newborn rats were shortened,the number of times crossing platforms was increased,pathological damage in hippocampal CA1 region was reduced,the numbers of syn-apses and synaptic vesicles increased,and the thickness of postsynaptic dense matter thickened,the levels of MDA,neuronal apoptosis rate,Bim,p53,p-p38 MAPK,and p-ERK proteins in the brain tissue of the hippocampal CA1 region decreased,the level of SOD increased in the lidocaine group,the trend of corresponding indexes in the animomycin group was opposite that in the lidocaine group(P＜0.05).Anisinomycin reversed the protective effect of lidocaine on neonatal rats in the delivery as-phyxia group.Conclusion Lidocaine-induced labor analgesia improves neonatal hypoxia by inhibiting the MAPK/ERK sig-naling pathway.
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