Isolation and Identification of Pathogen of Strawberry Root Rot in Nanjing Area
Objective:Exploring the pathogenic bacteria of strawberry root rot in strawberry base,Lishui District,Nanjing City,Jiangsu Province.Methods:The isolated strains were determined by morphologi-cal identification and re-experiments.The pathogenic strains were identified by multigene sequencing with inner transcriptional spacer(ITS),actin genes(ACT),glyceraldehyde 3-phosphate dehydrogenase gene(GAPDH),β-tubulin gene(TUB2),and chalcone synthase gene(CHS).Results:Disease plants were collected from strawberry bases and 20 strains were obtained by tissue isolation.Three pathogenic strains NJLS2,NJLS6,and NJLS7 were identified by re-experiments.Based on the multigene combined sequencing and the morphological characteristics identification,Colletotrichum siamense was the patho-genic bacteria causing the strawberry root rot.Conclusion:This study provides a theoretical basis for ana-lyzing the biodiversity of strawberry root rot pathogens and has practical significance to the efficient pre-vention and control of strawberry root rot in Lishui strawberry base.
NETL
NSTL
万方数据
维普
