Objective:The differences in anthocyanin content and photosynthetic characteristics of dif-ferent leaf colors of Cinnamomun bodinieri were compared,and the mechanism of leaf color formation in spring was analyzed preliminarily.Methods:The leaf anthocyanin content,chlorophyll content,chlorophyll fluorescence parameters,photosynthetic parameters and the expression of anthocyanin syn-thesis-related genes were determined in three C.bodinieri seedlings with different leaf colors.Results:The content of chlorophyll a,chlorophyll b,chlorophyll(a + b),and carotenoid in purple and red leaf seedlings was not significantly different from that in green seedlings,but the content of anthocyanin in leaves of purple and red seedlings was 134.24 and 49.80 times higher than that of green seedlings,re-spectively.The net photosynthetic rate(Pn),stomatal conductance(Gs)and transpiration rate(Tr)of red and purple leaf seedlings were higher than those of green leaf seedlings,while the intercellular CO2 concentration(Ci)and water use efficiency(WUE)were lower than those of green leaf seedlings.In terms of photosystem function,the initial fluorescence(Fo),the maximum fluorescence under dark ad-aptation(Fm),the nonphotochemical quenching coefficient(NPQ),and the maximum quantum yield of PSⅡ under light adaptation(Fo'/Fm')of red and purple leaf seedlings were higher than those of green leaf seedlings,but the photochemical quenching coefficient(qP)and the actual photochemical efficien-cy(ΦPSⅡ)were lower than those of green leaf seedlings,and the significant difference was not found-ed about the maximum photochemical efficiency of PSⅡ under dark adaptation(Fv/Fm)in three C.bodinieri seedlings.The CbDFR,CbANS and CbUFGT gene expression levels of the purple leaf seed-lings were significantly higher than those of the green leaf seedlings.The CbDFR,CbANS and CbUF-GT gene expression levels of the red leaf seedlings were higher than those of the green leaf seedlings,and only CbUFGT was significantly different.Conclusion:Anthocyanin was regarded as the main pig-ment of purple and red color in leaves of C.bodinieri,and has protective function on photosynthetic system of C.bodinieri leaves.CbDFR,CbANS and CbUFGT were regarded as the key genes for color rendering in C.bodinieri leaves.
维普
万方数据
