Objective:In order to explore the response mode of rhizosphere soil microorganisms to the planting mode of Chinese medicinal materials.Methods:The research objects were intercropping As-tragalus membranaceus and monocropping Angelica sinensis rhizosphere soil.Based on high-through-put sequencing technology,the differences in community composition and function of soil microorgan-isms in different seasons and planting patterns were analyzed.Results:The results showed that the Chao1 index and ACE index under intercropping were higher than those of monocropping.Proteobacte-ria,Acidobacteria,Actinobacteria,Chloroflexi and Gemmatimonadetes were the dominant phylums under intercropping,and the relative abundance of Gemmatimonadetes under intercropping was higher than that under monocropping.The abundance of γ-Proteobacteria in November and the Homophage in July were significantly higher than that in monocropping.KEGG function prediction showed significant differences in the relative abundance of 23 secondary functional genes under rhizosphere soil bacteria and cell processes,environmental information processing,genetic information processing,and metab-olism under different time and intercropping conditions.The OTU numbers of functional genes K00371(narH/narY/nxrB)and(K00374 narI/narV)involved in nitrogen cycling were significantly higher than those in monocropping.Intercropping co-occurrence networks of different phylums and classes are more closely connected and complex than those under monocropping.Conclusion:Angelica sinen-sis intercropping Astragalus membranaceus affects soil bacterial community composition,increase its richness and functional richness,affects soil nitrogen cycling capacity,and promotes soil microbial in-teractions.
维普
万方数据
