Objective:Cypripedium tibeticum was a typical mycorrhizal plant and also an endangered plant.To study the symbiotic relationship between mycorrhizal fungi and C.tibeticum,and provide a theoretical basis for its conservation.Methods:The level of mycorrhizal fungal diversity of C.tibeti-cum and its probiotic effect were studied by using microbial pure culture and rewiring methods.Results:A total of 366 fungal strains were isolated from the roots of four populations of C.tibeticum,involving seven genera:Tulasnella,Rhizoctoia,Fomes,Chaetomium,Ceratobasidium,Corticium and Epulorhiza.At the genus classification level,the dominant group was the genus Tulasnella with the highest isolation rate(15.88%)and frequency of isolation(34.70%),followed by the genus Rhizoc-toia.The mycorrhizal fungal composition,dominant groups and isolation frequencies of different popu-lations of C.tibeticum differed.Among them,Tulasnella,Rhizoctoia and Fomes were the common fun-gi of C.tibeticum.All seven test strains were infested the seedlings of C.tibeticum,and five of them(CT-1-64,CT-1-28,CT-3-13,CT-4-87 and CT-2-06)were promoted seed germination and seed-ling growth.We also screened and obtained an efficient growth-promoting strain CT-1-64,which can improve the seed germination process and the fresh weight rate and nutrient content of seedlings.Conclusions:The presence of abundant and diverse types of mycorrhizal fungi in C.tibeticum promotes seed germination and seedling growth,and the symbiotic relationship of efficient growth-promoting fungi needs to be further investigated.
维普
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