Objective:To evaluate the quality of Gnaphalium affine D.Don.Methods:The qualitative identification was performed by TLC.The contents of water,total ash,acid-insoluble ash and extract were determined according to the methods of the four general rules of the Chinese Pharmacopoeia(2020 edition).The contents of luteolin and apigenin were determined by HPLC.Cluster analysis and principal component analysis were performed on 23 batches of G.affine by SPSS 20.0.Results:The TLC spots were clear with good resolution.The content of water,total ash,acid-insoluble ash and ex-tract were 7.83%～10.70%,5.95%～17.11%,0.83%～9.40%and 8.87%～27.43%in 23 batches of samples,respectively.Luteolin and apigenin showed good linear relationships within the ranges of 0.7～374.5 μg/mL(r = 0.999 9)and 0.4～195.5 μg/mL(r = 0.999 4),whose average recoveries were 98.95%(RSD = 1.94%)and 97.18%(RSD = 3.46%),respectively.The samples from Tibet were clus-tered into one category,while the others were distributed discretely.The classification results were re-lated to regions.Conclusion:This stable and reliable method could be used for the quality evaluation of G.affine.
维普
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