首页|基于多指标结合化学计量学的多基源藏药烈香杜鹃质量评价分析

基于多指标结合化学计量学的多基源藏药烈香杜鹃质量评价分析

Quality Evaluation and Analysis of Multi-base Source of Tibetan Medicine Flos Rhododendri Anthopgonoidi Based on Multi-index Combined Chemometrics

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目的:采用多指标结合化学计量学综合评价烈香杜鹃的质量,为其质量评价提供科学依据.方法:采用2020年版《中国药典》四部通则规定的方法对藏药烈香杜鹃的显微、水分、总灰分等分别进行了鉴别和测定,采用高效液相色谱法(HPLC)测定了金丝桃苷和槲皮苷的含量,并进行聚类分析(HCA)和主成分分析(PCA).采用中药色谱指纹图谱相似度评价系统(2012)进行了相似度评价.结果:不同基源烈香杜鹃的显微鉴别结果存在细微差别,水分、总灰分、酸不溶性灰分、浸出物含量也显示出一定的差异.指纹图谱的相似度分析显示样品相似度系数为0.705～0.990.金丝桃苷和槲皮苷的含量平均值分别为0.408 2%、0.175 5%、0.583 7%,RSD值分别为62.97%、46.67%和47.89%.化学模式分析结果显示样品间存在一定差异.结论:不同基源的烈香杜鹃质量有一定的差异性,基于多指标结合化学计量法的方法能有效评价藏药烈香杜鹃的质量,为烈香杜鹃的质量评价提供参考.
Objective:To evaluate the quality of Flos Rhododendri Anthopgonoidi by multi-index com-bined with chemometrics,so as to provide scientific basis for quality evaluation.Methods:The micro-scopic content,moisture,total ash of Flos Rhododendri Anthopgonoidi were identified and determined by the methods specified in the four general rules of Chinese Pharmacopoeia(2020 Edition).Hypericin and quercetin contents were determined by high performance liquid chromatography(HPLC),and hi-erarchical cluster analysis(HCA)and principal component analysis(PCA)were performed.In addi-tion,this study also evaluated the similarity of Flos Rhododendri Anthopgonoidi by similarity evalua-tion of chromatographic fingerprint of Traditional Chinese Medicine(2012).Results:There were slight differences in the microscopic identification results of multi-base source of Flos Rhododendri An-thopgonoidi,the moisture,total ash,acid insoluble ash and extract of multi-base source of samples were not consistent,either.The similarity analysis of fingerprints showed that the similarity coefficient of 20 batches of samples ranged from 0.705 to 0.990.And the mean contents of hypericin and quercitrin were 0.408 2%,0.175 5%,0.583 7%,and RSD were 62.97%,46.67%and 47.89%,respectively.Chemical model analysis showed that there were differences among the samples.Conclusion:There was a certain degree of variability in the quality of multi-base sources of Flos Rhododendri Anthop-gonoidi.The method could effectively evaluate the quality of the Tibetan medicin,and provide a refer-ence for the quality evaluation of Flos Rhododendri Anthopgonoidi.

Flos Rhododendri AnthopgonoidiMulti-base sourceChemometricsQuality evalua-tionIdentification of medicinal materials

周莹莹、刘翔、朱可欣、舒抒、邱毅、兰钧、阳勇、张小梅、王森

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遵义医科大学 药学院,贵州 遵义 563000

重庆市中药研究院,重庆 400065

西藏自治区食品药品检验研究院 国家药品监督管理局中药(藏药)质量控制重点实验室 西藏 拉萨 850000

烈香杜鹃 基源 化学计量学 质量评价 生药鉴定

中医药现代化研究重点专项中国药品监管科学行动计划重庆市基本科研业务费项目重庆市基本科研业务费项目

2019YFC1712300NMPAJGKX-2023-081cstc2023jxjljbkyX0002csct2021jxjijbky10003

2024

10.3969/j.issn.1006-9690.2024.03.001

中国野生植物资源
中华全国供销合作总社南京野生植物综合利用研究院

中国野生植物资源

影响因子：0.667
ISSN：1006-9690
年,卷(期)：2024.43(3)
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