响应面法优化超声辅助提取蕨麻总三萜工艺研究
Optimization of Ultrasonic-assisted Extraction of Total Triterpenoids from the Roots of Potentilla anserina by Response Surface Methodology
岳洪霞 1胡娜 2隆海燕 1王洪伦 2栾广祥1
作者信息
- 1. 青海大学 医学部药学系,青海 西宁 810001
- 2. 中国科学院 藏药研究重点实验室,青海 西宁 810008
- 折叠
摘要
目的:优化超声辅助提取蕨麻总三萜工艺条件研究.方法:在单因素试验基础上,利用响应面法评估液料比、提取时间、提取温度对总三萜提取得率的影响.结果:超声辅助提取最佳工艺参数为液料比20 ∶ 1 mL/g,提取时间63 min,提取温度60℃,蕨麻总三萜提取得率可达4.73%±0.01%.结论:响应面法优化提取工艺切实可行,操作简单,总三萜含量高,可为蕨麻进一步开发利用提供依据.
Abstract
Objective:This study optimizes the extraction conditions for total triterpenoids from Poten-tilla anserina roots using ultrasonic-assisted extraction.Method:After conducting single-factor experi-ments,the effects of liquid-solid ratio,extraction time,and extraction temperature on the extraction rate of total triterpenoids was assessed through response surface methodology.Results:The optimal ex-traction parameters with the environment of a liquid-solid ratio of 20 ∶ 1 mL/g,an extraction time of 63 min,and an extraction temperature of 60°C.Under these conditions,the extraction rate of total trit-erpenoids from P.anserina roots reached 4.73%±0.01%.Conclusion:The extraction process was sim-plified and optimized using the response surface method,resulting in a high content of total triterpe-noids.This optimization provides a foundation for the future development and utilization of P.anserina root extracts.
关键词
蕨麻/总三萜/超声辅助提取/响应面法Key words
The roots of Potentilla anserina/Total triterpenoids/Ultrasonic-assisted extraction/Re-sponse surface methodology引用本文复制引用
基金项目
青海省科技厅基础研究青年项目(2022-ZJ-945Q)
出版年
2024
NETL
NSTL
万方数据