Objective:To select the best of 4 varieties of Astragalus membranaceus var.mongholicus.Methods:A single-factor randomized block design was used to study the differences of photosynthetic characteristics,antioxidant enzyme activity,yield and quality of different A.membranaceus var.mong-holicus varieties,which provided support for the promotion of high-quality varieties of A.membrana-ceus var.mongholicus.Results:The results showed that the levels of chlorophyll b content,carotenoid content,peroxidase(POD)activity,yield,astragalus polysaccharide and extract content were ranked as Longqi-3>Longqi-1>Longqi-2>Longqi-4.The order of net photosynthetic rate and calycosin-7-glucoside content in A.membranaceus var.mongholicus was Longqi-3>Longqi-1>Longqi-4>Longqi-2.The order of stomatal conductance,astragaloside content and catalase(CAT)activity was Longqi-3>Longqi-2>Longqi-1>Longqi-4.The transpiration rate ranked as Longqi-2>Longqi-3>Longqi-1>Longqi-4.The intercellular CO2 concentration ranked as Longqi-3>Longqi-4>Longqi-1>Longqi-2.The chlorophyll a content ranked as Longqi-1>Longqi-3>Longqi-2>Longqi-4.The SOD activity ranked as Longqi-1>Longqi-2>Longqi-3>Longqi-4.Conclusion:Under the conditions of this experiment,Longqi-3 had the best quality,with the highest yield and ac-tive ingredient content.Longqi-3 was suitable for promoting cultivation in Weiyuan County,which could increase the income of local farmers and promote the development of local economy.