Objective:The best harvest time of Ziziphi Spinosae Semen(ZSS)was analyzed compre-hensively from the kernel rate,oil yield and thirteen main chemical components content,and the quali-ty markers of the best harvest time was determined.Methods:The Ziziphus jujuba Mill.var Spinosa(Bunge)Hu ex H.F.Chou was harvested from August 28 to October 17,kernel rate and oil yield were calculated.GC-MS and HPLC-UV-ELSD methods were used to determine the changes of chemical constituents in ZSS at different harvesting periods.Multivariate statistical analysis was used to screen the different components of ZSS at different harvesting stages,and the corresponding targets and path-ways of the different components were found through network pharmacology.Results:The kernel rate and oil yield of ZSS harvested on September 30 reached the highest value.The contents of primary me-tabolites(fatty acids)and secondary metabolites(alkaloids,saponins and flavonoids)of ZSS were the highest from September 16 to October 17,and the best harvesting time for ZSS was finally determined from September 16 to October 17.By using multivariate statistical analysis,network pharmacologic analysis and the principle of combining quality markers,the five components of linoleic acid,oleic ac-id,coclaurine,jujuboside A and jujuboside B were predicted to be the quality markers of the best har-vest time of ZSS.Conclusion:The study established a multi-index comprehensive analysis method for the quality of ZSS in different harvesting periods,and determined the quality markers for the study of the best harvesting period of ZSS,which provided references for the quality control of ZSS.
维普
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