Objective:To investigate the effect of oleanolic acid saponins from Pfaffia glomerata ex-tract on the synthesis and secretion of androgen in TM3 mouse Leydig cells,and screen out the compo-nents with strong activity according to the cell test.Methods:Two kinds of macroporous adsorption res-ins(AB-8 and XAD1600)were used to separate and enrich different components as test materials.Methyl thiazolyl tetrazolium(MTT)method and enzyme-linked immunosorbent assay were used to de-termine the effects of different components and concentrations on TM3 cell activity,testosterone(T)content,dihydrotestosterone(DHT)content and cyclic guanosine monophosphate(cGMP)content.Nitric oxide(NO)content,superoxide dismutase(SOD)activity and malondialdehyde(MDA)con-tent of TM3 cells were detected by nitrate reduction method,hydroxylamine method and thiobarbituric acid method,respectively.Results:The 70%ethanol fraction isolated by AB-8 macroporous adsorp-tion resin had a stronger activity in promoting the secretion of androgen in TM3 cells than other frac-tions.The 70%ethanol fraction was further purified and enriched by XAD1600 macroporous adsorp-tion resin.The obtained GP-1 and GP-2 fractions significantly promoted the secretion of T,DHT and cGMP in TM3 cells(P＜0.05,P＜0.01),increased the activity of SOD enzyme(P＜0.01)and re-duced the content of MDA(P＜0.05,P＜0.01),and the effect of GP-2 fraction was better than that of GP-1 fraction.Conclusion:The synthesis and secretion of androgen and antioxidant capacity of TM3 cells were significantly improved by separation and purification components of P.glomerat.Among them,the activity of GP-2 component was stronger,and its main active component was olea-nolic acid saponin.
Pfaffia glomerataSaponinPurification and enrichmentMouse Leydig cellsAndrogen
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