Objective:Primulina hunanensis was a unique karst cave wild plant with great ornamental value in China.It had the characteristics of shade tolerance and barren tolerance,but its natural distribu-tion range was narrow and the size of wild population was small.Embryonic development was a key link in the life history of plants.Studying the embryonic development of P.hunanensis would provide a new idea for the cultivation and breeding of plants in Gesneriaceae.Methods:Using P.hunanensis as the material,conventional paraffin sectioning technology was used to make anatomical observations on the megasporogenesis,microsporogenesis and the development of female and male gametophytes.Re-sults:The anthers of P.hunanensis had four chambers,and the anther wall from outside to inside was sequentially composed of epidermis,endothecium,middle layer and glandular tapetum.The cytokine-sis type was simultaneous type during meiosis of the microspore mother cell.A few tetrads were decus-sate type,most were tetrahedral type,and the pollen was two-celled type.The ovules were anatro-pous,single integument,tenuinucellate ovule,and the mode of development of the embryo sac was polygonum type.The pollen grain development of P.hunanensis was not synchronized,and there were phenomena of pollen abortion,male and female gametophyte heterogenesis.Conclusion:The study ac-cumulated basic embryological data for plants of the genus Primulina,which was of great significance for revealing the reproductive mechanism of plants in Gesneriaceae and improving plant reproductive breeding methods.
维普
万方数据
