Objective:The composition and correlation of endophytic fungi in the roots and rhizosphere soil of Cypripedium macranthos was analyzed to provide theoretical basis for the field population recov-ery and sustainable resource utilization.Methods:The nuclear gene ITS2 fragment of endophytic fungi in the roots and rhizosphere soil was amplified and sequenced by high-throughput sequencing.Results:Except for a few samples,the number of endophytic fungi in OTU,genus,family,order,class and phylum was significantly different in roots and rhizosphere soil,and the number of endophytic fungi in all rhizosphere soil samples were larger than that in root samples.There was no common OTU and ge-nus among the different samples.The results of Alpha diversity showed that the richness of fungal com-munity in rhizosphere soil was higher than that in root samples,but the difference in diversity was not significant.At the family level,there were no common dominant fungi in root and rhizosphere soil.The results of PCoA principal component analysis and UPGMA cluster analysis showed that there were differences in composition between root and rhizosphere soil samples.In terms of the specific composi-tion of fungi,Rozellomycota was relatively close in abundance and relatively uniform in root samples and rhizosphere soil samples.Malassezia was only detected in root samples and was endemic to root samples.Sebacina was only detected in rhizosphere soil and was endemic to rhizosphere soil.Conclu-sion:The endophytic fungal community composition of roots and rhizosphere soil was different.The abundance of endophytic fungi in rhizosphere soil was higher than that in root samples.The correlation between the two fungal communities was small,and there was no common dominant fungi.
Cypripedium macranthosFungi in the rootRhizosphere soilHigh-throughput se-quencing
维普
万方数据
