首页|低共熔溶剂提取酸枣果肉多糖工艺及其对α-葡萄糖苷酶抑制活性研究

低共熔溶剂提取酸枣果肉多糖工艺及其对α-葡萄糖苷酶抑制活性研究

Deep Eutectic Solvent Extraction Polysaccharide of Wild Jujube Flesh and Research on Its Inhibitory Activity against α-Glucosidase

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目的:为提高酸枣果肉的利用率,利用低共熔溶剂(DES)提取酸枣果肉多糖(WJFP)并优化其提取工艺,探究WJFP对α-葡萄糖苷酶抑制活性.方法:采用单因素和响应面实验以多糖得率为指标筛选最佳提取工艺,利用HPAEC分析其单糖组成并测定WJFP对α-葡萄糖苷酶抑制活性.结果:最佳DES体系为氯化胆碱-尿素(CCU),摩尔比为1∶2;最佳提取工艺为超声时间62 min、超声温度54℃、含水量24%、料液比1∶20(g·mL-1),在此条件下,WJFP得率可达5.57%;单糖组成分析得阿拉伯糖和半乳糖醛酸为WJFP主要单糖;0.2 mg·mL-1 WJFP对α-葡萄糖苷酶的抑制活性可达到82.05%.结论:利用DES提取所得WJFP对α-葡萄糖苷酶具有一定的抑制活性,拓宽了酸枣果肉的开发前景.
Objective:To improve the utilization rate of wild jujube flesh,deep eutectic solvent(DES)was used to extract polysaccharides from wild jujube flesh(WJFP)and optimize the extraction pro-cess.The α-glucosidase inhibitory activity of the polysaccharides was also determined.Methods:The optimal extraction process was explored through single factor and response surface experiments.The monosaccharide composition was determined by HPAEC,and the α-glucosidase inhibitory activity of WJFP was measured.Results:The optimal DES system was choline chloride-urea(CCU)with a mo-lar ratio of 1∶2.The optimal extraction process parameters were as follows:ultrasonic time of 62 min,ultrasonic temperature of 54℃,moisture content of 24%,and solid-liquid ratio of 1∶20(g·mL-1).Under these conditions,the yield of WJFP reached 5.57%.Analysis of WJFP monosaccha-ride composition showed that arabinose and galacturonic acid were the main monosaccharides in the polysaccharides.The α-glucosidase inhibition activity of WJFP at a concentration of 0.2 mg·mL-1 reached 82.05%.Conclusion:Polysaccharides extracted using DES from wild jujube flesh exhibited significant α-glucosidase inhibitory activity,expanding the development prospects of wild jujube flesh.

Wild jujube fleshDeep eutectic solventsPolysaccharideResponse surfaceα-Gluco-sidase

袁书会、陈强、刘红波、史鑫波、李国龙

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陕西中医药大学 陕西中药资源产业化省部共建协同创新中心/秦药特色资源研究开发国家重点实验室(培育),陕西 咸阳 712083

酸枣果肉 低共熔溶剂 多糖 响应面法 α-葡萄糖苷酶

陕西省教育厅重点科学研究计划项目陕西中医药大学博士科研启动金项目

22JY02017102032238

2024

中国野生植物资源
中华全国供销合作总社南京野生植物综合利用研究院

中国野生植物资源

影响因子:0.667
ISSN:1006-9690
年,卷(期):2024.43(7)
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