首页|潞党参中低聚糖的分离纯化及其抗炎活性研究

潞党参中低聚糖的分离纯化及其抗炎活性研究

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目的:分析低聚糖的成分构成及其在潞党参中的抗炎作用.方法:采用DEAE-52阴离子纤维素和Sepha-dex G-15葡聚糖凝胶色谱柱分离纯化低聚糖组分.利用体外细胞实验、Griess法、Western blot检测法检测其抗炎活性.结果:得到了相对分子量为1 452 Da的低聚糖组分D1,得率为0.13%±0.05%,蛋白杂质含量为(31.08±1.25)μg/mg,纯度为96.89%.单糖组成研究表明低聚糖D1主要由葡萄糖(63.40%)、阿拉伯糖(30.06%)和甘露糖(6.54%)组成.体外细胞实验表明,低聚糖D1样品在25～400 μg/mL浓度范围对RAW 264.7巨噬细胞无细胞毒作用.在LPS诱导的RAW 264.7巨噬细胞炎症模型中,低聚糖D1能有效地抑制炎症因子NO的释放.Western blot检测发现,低聚糖D1样品的共孵育处理能剂量依赖性抑制iNOS蛋白的表达.结论:潞党参中低聚糖组分D1能通过抑制炎症介质NO的释放,起到良好的抗炎作用.
Study on Separation,Purification and Anti-inflammatory Activity of Oligosaccharides from Lu Dangshen
Objective:Exploring the composition and anti-inflammatory activity of oligosaccharides in Lu Dangshen.Methods:The oligosaccharide components were separated and purified using DEAE-52 anionic cellulose and Sephadex G-15 dextran gel column.The anti-inflammatory activity was detected by in vitro cellular assay,Griess method,and Western blot assay.Results:The oligosaccharide compo-nent D1 with a relative molecular weight of 1 452 Da was isolated and purified.The yield was 0.13%±0.05%,the protein impurity content was(31.08±1.25)μg/mg,and the purity was 96.89%.The mono-saccharide composition study showed that D1 was mainly composed of glucose(63.40%),arabinose(30.06%)and mannose(6.54%).The anti-inflammatory activity of D1 was explored,and it was found that D1 sample had no cytotoxic effect on RAW 264.7 cells in the concentration range of 25～400 μg/mL.In LPS-induced RAW 264.7 cell inflammation model,D1 could inhibit the release of NO.Western blot assay revealed that co-incubation treatment of D1 samples dose-dependently inhibited iN-OS protein expression.Conclusion:Oligosaccharide component D1 of Lu Dangshen could inhibit the release of inflammatory factor NO and played a good anti-inflammatory role.

Lu DangshenOligosaccharideSeparation and purificationPhysicochemical propertyAnti-inflammatory activity

汤敏、陆姗姗、谷巍、胡守芳

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南京中医药大学 药学院,江苏 南京 210023

南京中医药大学翰林学院,江苏 泰州 225300

南京市浦口区中医院,江苏 南京 211800

潞党参 低聚糖 分离纯化 理化性质 抗炎活性

国家中医药管理局道地药材生态种植及质量保障项目江苏省林业科技创新与推广项目泰州市社会发展项目泰州市社会发展项目大学生创新创业训练项目大学生创新创业训练项目江苏省高等学校自然科学面上项目

012072003039-1LYKJ-常州[2020]04SSF20220031SSF20230025202313981013Y202313981008Y23KJD360003

2024

10.3969/j.issn.1006-9690.2024.07.002

中国野生植物资源
中华全国供销合作总社南京野生植物综合利用研究院

中国野生植物资源

影响因子：0.667
ISSN：1006-9690
年,卷(期)：2024.43(7)
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