Trichosanthes kirilowii was an important medicinal plant with various medicinal values and wide application prospects.Traditional cultivation methods faced challenges such as low efficiency and unstable quality.The rapid growth and reproduction of T.kirilowii aseptic seedling could be successful-ly achieved through tissue culture.Furthermore,the yield and quality of T.kirilowii could be further im-proved through scientific and rational reproduction methods,water and fertilizer management,as well as disease and pest control.In this paper,the research progress of tissue culture rapid propagation and efficient cultivation techniques of T.kirilowii were reviewed in order to provide effective theoretical support for the production of T.kirilowii and promote the high-quality development of the T.kirilowii industry.