Objective:To optimize the extraction conditions of flavonoids from the leaves of Chimono-calamus fimbriatus with complex enzyme assistance and evaluate their potential for in vitro anti-diabe-tes activity.Methods:The response surface methodology was used to optimize the ultrasonic-coordi-nated complex enzyme extraction technology of flavonoids from the leaves.The total flavonoid yield of the extract and its extract components were used as the evaluation index,and the inhibitory activities of flavonoids on α-glucosidase and aldose reductase were studied.Results:The optimal extraction condi-tions were as follows:solid-liquid ratio 1∶24,ratio of the complex enzyme(pectinase∶cellulase)1∶3,hydrolysis temperature 51℃,and pH 6.0.Under the optimal conditions,the yield of flavonoids was 8.95% .The total flavonoid content of the aqueous phase extraction component of the crude extract was 1.175 mg/mL.The bioactivity studies showed that the α-glucosidase inhibitory activity of ethyl ac-etate extract and dichloromethane extract was 4.14 and 9.70 times higher than that of acarbose.At the same time,the dichloromethane extract had the strongest aldose reductase inhibitory activity(IC50=0.167 mg/mL),indicating that this solvent could better enrich the active components of these two en-zymes in the leaves.Pearson correlation analysis showed that flavonoids were the main active compo-nents in the leaves,and the inhibitory activities of α-glucosidase and aldose reductase were highly cor-related.Conclusion:The flavonoids of C.fimbriatus leaves have great potential in anti-diabetes drugs or health products.
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