Objective:The preparation technology of Broussonetia papyrifera leaves ointment and the determination method of the contents of chlorogenic acid,cryptochlorogenic acid,and scutellarin in the ointment preparation were established.Methods:The B.papyrifera leaves ointment preparation was prepared by emulsification method,and the contents of chlorogenic acid,cryptochlorogenic acid,and scutellarin were simultaneously determined by HPLC.The chromatographic column was Agilent ZOR-BAX SB-C18,and the column temperature was set to 40℃,acetonitrile-0.4% phosphoric acid aqueous solution was used as the mobile phase,gradient elution was performed,the flow rate was 0.8 mL/min,the injection volume was 10 μL;the detection wavelength was 339 nm.Results:The prepared B.papyr-ifera leaves ointment was easy to apply,remained stable without layering,and had a smooth texture without a grainy feeling.The chlorogenic acid,cryptochlorogenic acid and scutellarin contained in the corresponding concentration range respectively showed a good linear relationship(R2>0.999);the sample recovery rates of each target substance in the B.papyrifera leaves ointment ranged from 99.81% to 103.22%,and the relative standard deviations(RSD)were all<2% .Conclusion:The preparation process of B.papyrifera leaves ointment was stable and feasible.The HPLC method for the simultane-ous determination of its three components:chlorogenic acid,cryptochlorogenic acid,and scutellarin was found to be feasible.It demonstrated good accuracy and precision,and could be used for the quali-ty control of B.papyrifera ointment.
NETL
NSTL
万方数据
