Objective:To optimize the methods and conditions of in vitro culture of Atractylodes lancea and establish an efficient technology system for tissue culture and rapid propagation of A.lancea.Meth-ods:Using the sterile seedling of A.lancea as explants,the culture conditions were optimized,the best proliferation medium was screened,and the combination of in vitro and ex vitro was used to induce the root of the subculture seedlings of A.lancea to obtain the tissue culture seedlings.Results:The optimal hypocotyl cutting method was transection and hypocotyl preservation,with an average proliferation co-efficient of 3.80.The light intensity of 6 000 lx was more suitable for the induction of indefinite shoots,with an average proliferation coefficient of 4.36.The optimal culture medium for adventitious bud pro-liferation of A.lancea was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L+sucrose 50 g/L,the proliferation coefficient was 12.96±1.55,the highest proliferation coefficient of single bud could up to 15,and the uniformity of agronomic traits was the highest.In the process of rooting in ex vitro,the seedling cycle can be shortened by about 14 d compared with the rooting method in vitro.The optimum rooting sub-strates was peat∶perlite∶vermiculite=3∶1∶1.The average survival rate of ex vitro rooting was 95.00%,which was significantly higher than that of in vitro rooting.Conclusion:In this study,an in-novative technology system of tissue culture and rapid propagation of A.lancea was established by com-bining in vitro culture with ex vitro rooting technology,which could shorten the seedling cycle and im-prove the survival rate of A.lancea,and provide scientific and feasible technical support for the factory cultivation of endangered medicinal plant of A.lancea.
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