青海省海西州部分水域真核微藻资源调查与分析
Investigation and Analysis of Eukaryotic Microalgae Resources in Some Waters of Haixi Prefecture,Qinghai Province
杜少波 1鄂崇毅 2祁姝瑾 3谢惠春3
作者信息
- 1. 青海师范大学 地理科学学院,青海 西宁 810008;青海祁连山南坡森林生态系统国家定位观测研究站,青海 互助 810500
- 2. 青海师范大学 地理科学学院,青海 西宁 810008
- 3. 青海祁连山南坡森林生态系统国家定位观测研究站,青海 互助 810500;青海师范大学 生命科学学院,青海 西宁 810008
- 折叠
摘要
目的:探讨青海省海西州部分水域的真核微藻多样性特征.方法:采集察尔汗盐湖、茶卡盐湖、尕海盐湖、小柴达木盐湖、托素湖、可鲁克湖、都兰水库和雪山乡8个样点水域的样本,通过高通量测序分析其真核微藻群落结构组成和多样性.结果:8个样点中,共检测到501个OTU,分属于5门、23纲、52目、89科、139属,共同含有15个不同的OTU;8个样点所包含的绿藻门最多,其中CKYH的优势藻属为盐藻属,CEHYH的优势藻属为转板藻属、扁藻属和盐藻属,DLSK和TSH的优势藻属均为衣藻属和扁藻属,GHYH的优势藻属为衣藻属和Biecheleria,KLKH的优势藻属为扁藻属,XCDMYH的优势藻属为扁藻属、Mantoniella和衣藻属,XSX的优势藻属为扁藻属和盐藻属;Alpha多样性指数差异分析结果表示,KLKH和DLSK的丰富度最高,CEHYH、XSX、DLSK的多样性最高;PCoA分析显示,CKYH和CEHYH以及TSH和XCDMYH之间的群落结构组成较相似,KLKH和XSX以及XSX和DLSK之间的群落结构组成相似性较小,GHYH与其他7个样点的群落结构组成具有明显差异.结论:青海省海西州的真核微藻资源丰富,即使在盐湖、温泉水域等极端环境下,也蕴藏着十分丰富的真核微藻资源.研究结果对今后藻种的适应性进化机制研究、功能藻种的筛选等具有重要意义,有利于真核微藻资源的进一步开发和利用.
Abstract
Objective:To explore the diversity of eukaryotic microalgae in some waters of Haixi Prefec-ture,Qinghai Province.Methods:8 samples from Chaerhan Salt Lake,Chaka Salt Lake,Gahai Salt Lake,Xiaochaidamu Salt Lake,Tuosu Lake,Keluke Lake,Dulan reservoir and Xueshanxiang were collected,and the community structure and diversity of eukaryotic microalgae were analyzed by high-throughput sequencing.Results:In the 8 samples,501 OTUs were detected,which belonged to 5 phy-la,23 classes,52 orders,89 families,139 genera,and contained 15 different OTUs.The 8 sampling points contained the most Chlorophyta,among which CKYH's dominant algae genus was Dunaliella.CEHYH's dominant algae genera were Mougeotia,Tetraselmis,and Dunaliella.DLSK and TSH's dominant algae genera were both Chlamydomonas and Tetraselmis.GHYH's dominant algae genera were Chlamydomonas and Biecheleria.KLKH's dominant algae genus was Tetraselmis.XCDMYH's dominant algae genera were Tetraselmis,Mantoniella,and Chlamydomonas.And XSX's dominant al-gae genera were Tetraselmis and Dunaliella.The difference analysis of the Alpha diversity indices showed that KLKH and DLSK had the highest richness,while CEHYH,XSX and DLSK had the high-est diversity.PCoA analysis showed that CKYH and CEHYH,TSH and XCDMYH were similar in community structure.The similarity of community structure between KLKH and XSX,as well as XSX and DLSK was small.GHYH was significantly different from the other 7 samples.Conclusion:Eukary-otic microalgae resources were abundant in Haixi Prefecture,Qinghai Province,even in extreme envi-ronments such as salt lakes and hot spring waters.The research results were of great significance for the future study on the adaptive evolutionary mechanism of algae species and the screening of functional al-gae species,and were conducive to the further development and utilization of eukaryotic microalgae re-sources.
关键词
青海省海西州/真核微藻/群落结构组成/极端环境Key words
Haixi Prefecture,Qinghai Province/Eukaryotic microalgae/Community structure com-position/Extreme environment引用本文复制引用
基金项目
青海省"昆仑英才·高端创新创业人才"计划(领军人才)项目(1003-005024011)
&&(2023-ZJ-908M)
出版年
2024
NETL
NSTL
万方数据