Objective:To investigate the supercritical CO2 fluid extraction process of volatile compo-nents from the leaves of Pistacia weinmannifolia and their in vitro antioxidant activity.Methods:The volatile components were extracted by supercritical CO2 fluid,and the extraction process was opti-mized by single factor orthogonal experiment.The main components of volatile compounds were deter-mined by gas chromatography-mass spectrometry(GC-MS),and the antioxidant activities were eval-uated by the removal rates of DPPH,ABTS and OH free radicals.Results:The optimized extraction process for volatile components was as follows:extraction temperature of 30℃,extraction time of 45 min,extraction pressure of 24 MPa,and entrainer(absolute ethyl alcohol)dosage of 0.15 mL/g,with an extraction yield of 3.66% under these conditions.26,20 and 26 compounds were identified from the extracts using water,methanol and ethanol as entrainment agents,respectively.The main chemical components were terpenes,sterols,organic acids,ketenes and esters.The half-maximal clearance concentrations(IC50)of the volatile components extracted from P.weinmannifolia leaves on DPPH,ABTS and OH free radicals were 0.632,0.485 and 0.566 mg/mL,respectively.Conclusion:The optimized extraction process of volatile components from P.weinmannifolia leaves is reasonable and feasible,and the volatile components extracted under the conditions of this process have strong an-tioxidant activity in vitro.
Pistacia weinmannifolia leavesSupercritical CO2 extractionVolatile componentsAnti-oxidant activity
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