响应面法优选新疆藁本多酚超声提取工艺及抗氧化作用
Optimization of Ultrasound Extraction Process and Antioxidant Activity of Polyphenols from Conioselinum vaginatum by Response Surface Methodology
潘兰 1贾月梅 2周天源 1兰卫 1赵延龄1
作者信息
- 1. 新疆医科大学 中医学院,新疆 乌鲁木齐 830017
- 2. 新疆维吾尔自治区市场监督审核评价中心,新疆 乌鲁木齐 830000
- 折叠
摘要
目的:筛选新疆藁本多酚超声提取的最佳工艺,并对新疆藁本多酚的抗氧化能力进行评测.方法:以新疆藁本为原料,采用单因素法结合响应面优化法确定新疆藁本多酚最佳超声提取工艺,并用DPPH自由基清除率实验和Fe3+还原能力测试判断新疆藁本多酚抗氧化能力的强弱.结果:在25℃下,新疆藁本多酚超声提取的最优工艺为乙醇浓度62%,提取时间40 min,料液比1∶40(g⋅mL-1),此时多酚提取率为3.43%.新疆藁本多酚对DPPH自由基的清除能力和Fe3+的还原能力均随浓度增加呈上升趋势.新疆藁本多酚清除DPPH自由基的IC50是0.37 mg·mL-1.结论:本文建立的工艺验证试验结果与预测提取率接近,说明模型建立较好;新疆藁本多酚具备一定抗氧化能力,具有很好的开发应用前景.
Abstract
Objective:Screened the optimal process for ultrasonic extraction of polyphenols from Coni-oselinum vaginatum,and evaluated the antioxidant activity.Methods:The optimal ultrasonic extrac-tion process for C.vaginatum was determined by single factor method combined with response surface methodology(RSM).The antioxidant properties of C.vaginatum was evaluated by DPPH radical scav-enging activity and Fe3+reduction test.Results:The best operating experiment conditions for C.vagina-tum were as following:extraction time was 40 minutes,ethanol solutions was 62%,and the solid-liq-uid ratio was 1∶40(g⋅mL-1).Under the above conditions,the extraction yields of polyphenols from C.vaginatum was 3.43% .The scavenging ability of C.vaginatum polyphenols on DPPH free radicals and the redution ability of Fe3+exhibited an upward trend with the increase of concentration.The IC50 values of C.vaginatum for scavenging DPPH free radicals was 0.37 mg·mL-1.Conclusion:The estab-lished process validation test results were close to the predicted extraction rate,indicating that the mod-el was well established.Polyphenols from C.vaginatum had certain antioxidant potential and had a good prospects of development and application.
关键词
新疆藁本/多酚/响应面法/抗氧化Key words
Conioselinum vaginatum/Polyphenols/RSM/Antioxidant引用本文复制引用
基金项目
新疆自然科学基金项目(2020D01B48)
出版年
2024
NETL
NSTL
万方数据