中国野生植物资源2024,Vol.43Issue(10) :1-8.DOI:10.3969/j.issn.1006-9690.2024.10.001

基于HPLC指纹图谱及化学识别模式的炒山楂质量评价

Quality Evaluation of Fried Crataegus pinnatifida Fruit Based on HPLC Fingerprint and Chemical Pattern Recognition

段玺 汪芸兰 雷璇 魏玄 唐莹莹 宋逍
中国野生植物资源2024,Vol.43Issue(10) :1-8.DOI:10.3969/j.issn.1006-9690.2024.10.001

基于HPLC指纹图谱及化学识别模式的炒山楂质量评价

Quality Evaluation of Fried Crataegus pinnatifida Fruit Based on HPLC Fingerprint and Chemical Pattern Recognition

段玺 1汪芸兰 2雷璇 2魏玄 2唐莹莹 2宋逍3
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作者信息

  • 1. 陕西中医药大学附属医院,陕西 咸阳 712000
  • 2. 陕西中医药大学,陕西 咸阳 712046
  • 3. 陕西中医药大学,陕西 咸阳 712046;中药制药与新药开发教育部工程研究中心,北京 100029
  • 折叠

摘要

目的:为炒山楂中药饮片的质量评价提供参考.方法:采用"中药色谱指纹图谱相似度评价系统"(2012版)建立不同产地炒山楂的指纹图谱进行相似度评价,并对共有峰进行成分指认,将炒山楂成分含量数据输入SPSS软件进行聚类分析和主成分分析,并根据综合评分进行质量评价.结果:从建立的炒山楂指纹图谱中标定出5个共有峰,根据对照品图谱确定为5-羟甲基糠醛、新绿原酸、绿原酸、隐绿原酸和金丝桃苷;聚类分析将炒山楂样品分为4类;经主成分分析,提取出3个主成分,累计方差贡献率为90.433%,综合评分评价药材来源产地结果表明山东的炒山楂中药饮片质量较好.结论:本研究建立的炒山楂指纹图谱方法稳定、可靠,结合聚类分析、主成分分析对炒山楂的质量进行了多方面的综合评价,为炒山楂的质量评价提供了参考.

Abstract

Objective:To provide a reference for the quality evaluation of fried Crataegus pinnatifida fruit.Methods:The fingerprint chromatograms of fried C.pinnatifida fruit from different producing ar-eas were established using the"Similarity Evaluation System for Chromatographic Fingerprint of Tradi-tional Chinese Medicine"(2012 edition)for similarity assessment,and the components of the common peaks were identified.The data on the component contents of fried C.pinnatifida fruit were input into SPSS software for cluster analysis and principal component analysis,and quality evaluation was con-ducted based on comprehensive scores.Results:Five common peaks were identified from the estab-lished fingerprint chromatograms of fried C.pinnatifida fruit,which were identified as 5-hydroxymeth-ylfurfural,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid and hyperoside according to the reference spectrum.The fried C.pinnatifida fruit samples were divided into 4 categories by cluster analysis.Three principal components were extracted by principal component analysis,and the cumula-tive variance contribution rate was 90.433%.According to the comprehensive score,the quality of fried C.pinnatifida fruit from Shandong was better.Conclusion:The fingerprint chromatogram method of fried C.pinnatifida fruit established in this study was stable and reliable.Combined with cluster analy-sis and principal component analysis,the quality of fried C.pinnatifida fruit was comprehensively eval-uated from multiple aspects,which provided a reference for the quality evaluation of fried C.pinnatifi-da fruit.

关键词

炒山楂/化学模式识别/指纹图谱/聚类分析/主成分分析

Key words

Fried Crataegus pinnatifida fruit/Chemical pattern recognition/Fingerprint/Cluster analysis/Principal component analysis

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出版年

2024
中国野生植物资源
中华全国供销合作总社南京野生植物综合利用研究院

中国野生植物资源

CSCD
影响因子:0.667
ISSN:1006-9690
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