Objective:To investigate the genetic diversity and quality of Pueraria montana.Methods:The genomic DNA was extracted from young leaves of P.montana and AFLP molecular marker tech-nology was used to amplify DNA samples.The AFLP amplification results were analyzed by GeneMap-per software.High performance liquid chromatography(HPLC)was used to determine the content of isoflavonoids such as puerarin,daidzin,and daidzein in P.montana,and cluster analysis of compo-nent content was conducted using SPSS software.Results:Five pairs of AFLP fluorescent primers pro-duced 1113 polymorphic bands,with a polymorphism rate of 100%.Unweighted pair-group method with arithmetic means(UPGMA)cluster analysis indicated that sample 20(P.montana var.lobata from Chaling,Hunan)showed significant differences from other resources,separating from the other samples at a correlation coefficient of 0.35.The remaining samples were divided into two major clus-ters at a correlation coefficient of 0.48.One cluster mainly comprised P.montana var.lobata(from Yun-nan and Chongqing),but samples 17(P.montana var.thomsonii from Wugang,Hunan)and 18(P.montana var.thomsonii from Jiangbei,Hunan)clustered with this group,indicating similar genetic backgrounds to P.montana var.lobata.The other cluster primarily consisted of P.montana var.thomso-nii(from Guangdong,Guangxi,and Hunan)and P.montana var.lobata in Hunan,with Hunan P.montana var.lobata resources showing significant differences from P.montana var.lobata in Yunnan and other regions.The content analysis results indicated that P.montana var.lobata and P.montana var.thomsonii belonged to two distinct groups,with P.montana var.lobata having higher component con-tent than P.montana var.thomsonii.However,sample 17(P.montana var.thomsonii from Wugang,Hunan)clustered with P.montana var.lobata group,with high content of puerarin and daidzin.Conclu-sion:The genetic diversity of P.montana was revealed by AFLP molecular marker technology.There were significant differences in component content between P.montana var.lobata and P.montana var.thomsonii.Sample 17(P.montana var.thomsonii from Wugang,Hunan)exhibited similar component contents to P.montana var.lobata,highlighting it was an excellent P.montana resource.
维普
万方数据
