Objective:The leaf of Corydalis yanhusuo was used as explants to induce callus,the growth parameters and contents of alkaloids in the callus were measured,to provide a basis for the study of alkaloids metabolic regulation and superior varieties breeding of C.yanhusuo.Methods:The combination of 2,4-dichlorophenoxyacetic acid(2,4-D)and 6-benzyladenine(6-BA)were used for callus induction.The callus biomass was measured by weight method.Localization of alkaloids was performed by histochemical staining method.The contents of tetrahydropalmatine and dehydrocoryda-line were determined by high-performance liquid chromatography method.Results:The callus induc-tion rate of C.yanhusuo reached 75%by Murashige and Skoog medium with 2 mg/L 2,4-D and 1 mg/L 6-BA treatment.The callus was yellowish-white with a granular surface and grew rapidly.The fresh weight of subcultured callus was(2.7153±0.4398)g,with the multiplication coefficient of 4.43.The dry weight was(0.1552±0.0207)g,and the water content reached 94.16%.Callus parenchyma cells contained orange patches stained with Dragendorff's reagent,indicating the presence of alkaloids.The content of tetrahydropalmatine in the callus reached(0.8600±0.3502)mg/g DW(dry weight),meanwhile,dehydrocorydaline content reached(0.1744±0.0372)mg/g DW.Conclusion:The callus of C.yanhusuo induced by using leaves as explants showed a high induction rate and rapid growth,which was a suitable material for deriving alkaloids active ingredients such as tetrahydropalmatine quickly and stably.
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