Objective:To understand the current status of the germplasm resources of Gymnadenia,an in-depth investigation was conducted in the Sejila Mountain region of southeastern Xizang,focusing on the altitude range of 3000 m to 4600 m.Methods:By setting up sampling lines and plots,detailed records were made of the basic ecological information of Gymnadenia,including species composition,population quantity,associated plant species,vegetation types,and canopy density.Additionally,the geographic coordinates and altitudes were precisely recorded using GPS equipment.Results:Five natu-ral populations of Gymnadenia were discovered at different altitudes,identified as G.orchidis and G.crassinervis.Both species of Gymnadenia exhibited diverse variations in flower colors and morphology.The two species were found to prefer environments with ample sunlight and acidic soil rich in humus.The two species were observed to coexist within the specific altitude regions.The indi-viduals were distributed in a scattered and patchy manner within populations.Human excavation im-pacts were significant,and some populations were particularly severely affected.Furthermore,detailed descriptions of the habitat types and associated plant species of Gymnadenia were provided in this study.Conclusion:The germplasm resources of Gymnadenia in Sejila Mountain were facing severe threats and require immediate protection.This research provided a solid foundation for the conservation and sustainable utilization of wild resources of Gymnadenia,which were classified as second-class na-tional key protected plant species.