Objective:The extraction process of gentiopicroside from Gentiana apiata (GGA) was op-timized by response surface method,and the anti-acute liver injury activity of G. apiata was deter-mined. Methods:The factor levels were selected on the basis of single factor,and the response surface method was used to optimize the ultrasonic frequency,extraction time and solid-liquid ratio on the ex-traction of gentiopicroside from G. apiata. A mouse model of acute liver injury was induced by carbon tetrachloride(CCl4) to detect the levels of alanine aminotransferase (ALT) and aspartate aminotransfer-ase (AST) in serum,as well as the superoxide dismutase (SOD) activity,malondialdehyde (MDA) content,and liver weight in liver tissue. Results:The optimal extraction conditions for GGA were ultra-sonic frequency of 91 kHz,extraction time of 23 min,and solid-liquid ratio of 1:22. Under these conditions,the extraction yield of gentiopicroside was 0.328％,which was close to the predicted value of 0.344％. Compared with the model group,the high-and low-dose groups of G. apiata could signifi-cantly inhibit the increase of ALT and AST in the serum of mice (P<0.05),reduce the liver MDA con-tent,and enhance SOD activity (P<0.05). Conclusion:The response surface method can be used to optimize the extraction process of GGA. Under these conditions,the extract of G. apiata has the effect of anti-acute liver injury. The results of this study also provide a theoretical basis for the further devel-opment and utilization of G. apiata.
维普
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