Objective:To explore the mechanism of Euodiae Fructus in treating diarrhea based on net-work pharmacology and molecular docking technology,and predict the quality markers. Methods:Ac-tive components,target proteins of Euodiae Fructus,and diarrhea-related genes were retrieved and screened from multiple databases. The protein-protein interaction (PPI) network was constructed using the STRING database,and key targets were identified through network topology analysis with Cyto-scape software. Functional enrichment analysis of the PPI network,including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) signaling pathways,was performed on the Metascape platform. Furthermore,molecular docking was conducted using AutoDock software to eval-uate the binding affinities between active components and core targets. Finally,a "component-target-pathway" network was established to identify and predict the potential quality markers of Euodiae Fruc-tus for diarrhea treatment. Results:A total of 24 active components in Euodiae Fructus were screened,and these components correspond to 141 diarrhea-related targets. Twenty-four core targets,including TP53、AKT1、JUN、TNF and IL6,were obtained after the network topology analysis. GO and KEGG analysis showed that Euodiae Fructus may play an important role in the treatment of diarrhea by partici-pating in multiple pathways,such as cancer pathway and MAPK signaling pathway. Through molecu-lar docking and "quality marker-target-pathway" network,quercetin and β-sitosterol could be select-ed as quality markers of Euodiae Fructus in the treatment of diarrhea. Conclusion:Euodiae Fructus can treat diarrhea through multiple components,multiple targets and multiple pathways. Quercetin and β-sitosterol can be used as quality markers of Euodiae Fructus in the treatment of diarrhea. This results further improved the quality evaluation and control system of Euodiae Fructus.
维普
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