摘要
目的:考察和评价闽产重楼的药材品质.方法:通过实地调查和样品收集,采用高效液相色谱法测定样品中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ的含量.结果:闽产重楼有效成分以重楼皂苷Ⅶ为主,重楼皂苷Ⅰ、Ⅱ和Ⅵ为辅;7个产区中建瓯玉山种植的重楼有效成分含量最高,仙游菜溪的最低,合格率为71.43％;3～8年生重楼根茎有效成分累积总量在1.038～24.035 g范围内,以8年生累积总量最大,闽产重楼适宜采收年限可确定为8年;当年生长区段有效成分分别是去年、往年的1.47倍、1.83倍,往年的低于药典限量要求;不同大小重楼根茎有效成分与规格等级不完全成正比,仅凭根茎个体大小不足以评定药材商品等级.结论:重楼皂苷质量受产地、生长年限、生长区段和根茎大小的影响,闽产重楼品质良好、质量稳定.
Abstract
Objective:To examine and assess the quality of Paris polyphylla herbs cultivated in Fujian. Methods:After field surveys and sample collection,the levels of saponins Ⅰ,Ⅱ,Ⅵ and Ⅶ in P. poly-phylla were determined using high performance liquid chromatography. Results:P. polyphylla predomi-nantly contains saponin Ⅶ,supplemented by saponin Ⅰ,Ⅱ and Ⅵ. Among the seven production ar-eas,the highest content of active ingredients was observed in plants grown in Yushan Township,Ji-anou County,and the lowest in Caixi Township,Xiangyou County,with a pass rate of 71.43％. The variation in total accumulated active ingredients in P. polyphylla rhizome aged 3 to 8 years ranged from 1.038-24.035 g,with the maximum accumulation observed in 8-year-old plants. Based on these find-ings,the optimal harvesting age for P. polyphylla cultivated in Fujian appears to be 8 years. The active ingredient content of growth section in the current year was 1.47 times and 1.83 times of last year and previous years respectively,with the latter falling below the pharmacopoeial limit. The relationship be-tween active ingredient content and specification grade was not fully proportional across different rhi-zome size,indicating that rhizome size alone was insufficient to define the herb's commodity grade. Conclusion:The quality of saponins was influenced by the production areas,growth years,growth zones and rhizome size. Overall,the quality of P. polyphylla produced in Fujian is both favourable and stable.
维普
万方数据