Objective:This study aimed to reveal the diversity of endophytic microbiota in Drynaria roosii from Guizhou,and provide a basis for utilizing beneficial microbial communities to improve cul-tivation efficiency. Methods:Wild D. roosii tubers from Guizhou were used as materials. High-throughput sequencing and bioinformatics analysis were employed to analyze the diversity,network re-lationships,and functional prediction of endophytic microbial communities. Results:The D. roosii samples from 6 regions were classified into 39 classes and 646 genera of fungi,and 104 classes and 1007 genera of bacteria. The operational taxonomic units(OTUs) of fungi and bacteria were 49 and 1093,respectively. The main dominant fungi were Dothideomycetes、Sordariomycetes、Eurotiomycetes,while the dominant bacteria were Actinobacteria and Proteobacteria. Network analysis revealed a high positive correlation between most endophytic microbial genera. Among the D. roosii endophytic fungi,there were 24 genera of saprophytic fungi,20 genera of symbiotic fungi,9 genera of pathogenic fun-gi,4 genera of pathogenic-saprophytic fungi,and only 1 genus of pathogenic-symbiotic fungi. The highest proportions of various phenotypes in endophytic bacteria were aerobic KL(86.34％),anaerobic CS(7.73％),mobile element ZA(55.96％),facultative anaerobic WD(22.12％),biofilm-forming NY (22.36％),Gram-negative NY(55.41％),Gram-positive KL(75.75％),potentially pathogenic ZA (70.16％),and stress-tolerant WD(29.69％). Dactylonectria in the microbiota was commonly a patho-genic fungi for root rot. Variovorax may maintain the normal development of D. roosii roots by interact-ing with the rhizosphere environment. Sphingomonas may provide the necessary nutrients for the growth of D. roosii through nitrogen fixation,phosphate solubilization. And production of plant growth hormones to promote normal growth. Conclusion:The beneficial endophytic microbiota was of great significance for maintaining normal growth and development of D. roosii.And further research can be conducted to utilize these beneficial microbiota to improve the survival rate of D. roosii cultivation.
维普
万方数据
