盐旱胁迫对柳兰种子萌发及其幼苗生理特性的影响
Seed Germination and Seedling Physiological Characteristics of Chamerion angustifolium under Salt and Drought Stress
叶星 1李容榕 1林鹏程 1庄元文 1潘国庆 1胡星强 1张金魁 1周党卫 2吉美才让 3王环 4师生波4
作者信息
- 1. 青海民族大学 药学院,青海 西宁 810007
- 2. 青海民族大学 药学院,青海 西宁 810007;中国科学院 西北高原生物研究所 高原生物适应与进化重点实验室,青海 西宁 810008
- 3. 青海省海北州制药厂,青海 海晏 810200
- 4. 中国科学院 西北高原生物研究所 高原生物适应与进化重点实验室,青海 西宁 810008
- 折叠
摘要
目的:探究不同程度盐、旱胁迫下柳兰种子萌发特性和抗氧化酶活性的变化规律,旨在为柳兰的繁殖培育、园林栽培和药用价值的开发利用提供参考依据.方法:以柳兰种子为材料,分析了赤霉素(GA3)处理、干旱(PEG-6000)胁迫和盐(NaCl)胁迫对柳兰种子萌发的影响.结果:柳兰种子细小,表皮呈黄褐色,千粒重仅为(77.1±8.2)mg;GA3浓度为100～200 mg·L-1时,柳兰种子发芽率和发芽势均随GA3浓度升高而增加,但当浓度大于200 mg·L-1时,其发芽势和发芽率呈下降趋势.随着PEG-6000和NaCl浓度的增加,柳兰幼苗超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性显著升高,与胁迫程度呈极显著的正相关关系.结论:不同浓度NaCl和PEG-6000处理组间柳兰种子发芽率和发芽势差异显著(P<0.05),萌发率随着NaCl和PEG-6000浓度的升高逐渐下降,当NaCl浓度大于150 mmol·L-1或PEG-6000浓度大于15%时柳兰种子不能萌发.
Abstract
Objective:To explore the changes of Chamerion angustifolium seeds germination character-istics and antioxidant enzyme activities under different degrees of salt and drought stress,providing a reference basis for the propagation and cultivation of C.angustifolium,as well as the development and utilization of its landscaping and medicinal values.Methods:Using C.angustifolium seeds as experi-mental materials,the effects of gibberellin(GA3)treatment,drought(PEG-6000)stress and salt(Na-Cl)stress on the seeds germination of C.angustifolium were analyzed.Results:The seeds of C.angus-tifolium were small,with yellow-brown epidermis,and the weight of thousand grains was only(77.1±8.2)mg.When the GA3 concentration ranges from 100 to 200 mg·L-1,both the germination rate and germination potential of C.angustifolium seeds increased with the increase of GA3 concentration.How-ever,when the concentration exceeded 200 mg·L-1,the germination potential and the germination rate showed a tendency of decreasing.As the concentration of PEG-6000 and NaCl increased,the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)in C.angustifolium seedlings increased significantly,showing a highly significant positive correlation with the degree of stress.Con-clusion:The differences in germination rate and germination potential between different concentrations of NaCl and PEG treatments were significant(P<0.05).The germination rate of C.angustifolium seeds decreased gradually with the increase of NaCl and PEG concentrations.The seeds of C.angustifo-lium could not germinate when the concentration of NaCl exceeded 150 mmol·L-1 or the concentration of PEG-6000 exceeded 15%.
关键词
柳兰/赤霉素/盐胁迫/干旱胁迫/抗氧化活性Key words
Chamerion angustifolium/Gibberellin/Salt stress/Drought stress/Antioxidant activity引用本文复制引用
出版年
2024
NETL
NSTL
万方数据