Objective:To expand the population of Gleditsia japonica var.velutina and explore new methods for its reintroduction into the wild and artificially promoting natural regeneration.Methods:A field experiment simulating natural seeding was set up on the natural sloping land under the mother tree forest in a specific forest environment.The experiment was divided into three blocks,and each block containing three treatment sub-blocks with different planting depths,which were between semi-decom-posed litter layer and coarse humus layer,between coarse humus layer and humus layer,and 1 cm deep in humus layer.Each treatment block contained 30 planting points,with 2 seeds planted at each point.The nursery was used as the control group.Results:The germination rates of G.japonica var.ve-lution under simulated natural conditions were between 44.44%and 52.78%,with an average of 47.78%,which was higher than that of 35.00%in the nursery.The seedling rates were between 60.85%and 77.29%,with an average of 70.60%,which was lower than that of 95.00%in the nursery.The dif-ference between the germination rate and the seedling rate was not significant.The degree of human in-tervention affected the seedling rate and seedling growth.Conclution:In a specific forest environment,G.japonica var.velution can grow into seedlings by artificial simulation of natural planting,and a slightly higher germination rate can be achieved compared to conventional seedling raising in nursery.The horizontal complete litter layer and humus layer is the key to improve the germination rate of G.ja-ponica var.velution.