Pear was an important deciduous fruit tree crop in northern China,with wide distribution area and high commercial value.The construction of molecular markers played a vital role in pear genome research,gene mapped and marker-assisted breeding.In this paper,the research progress of molecular fingerprinting techniques in Pyrus plants was systematically reviewed.Especially,the applications of DNA markers in the following fields such as genetic diversity analysis,gene mapped construction and variety identification were also analysized.At the same time,the shortcomings of the current construction of DNA molecular fingerprints of Pyrus plants were explored as well as the future research focus was prospected.The information was helpful to properly use molecular marker technolo-gy to further construct molecular fingerprints of Pyrus plants and assist its breeding.