Objective To explore the effects of apigenin on apoptosis and hypoxia inducible factor-1α(HIF-1α)/nuclear factor κB(NF-κB)signaling pathway in renal cancer A498 cells.Methods Human renal cell carcinoma A498 cells were cultured in vitro and divided into different concentrations of apigenin(10,20,40 μmol/L)groups,apigenin(40 μmol/L)+HTF-1α agonist dimethylenediaminoacetic acid(DMOG)group,HIF-1α inhibitor rifiximab(YC-1)group,and control group.Cell proliferation was detected using cell counting kit-8(CCK-8)assay and plate clone formation assay,apoptosis was detected using Hoechst 33258 staining and flow cytometry,and expression of apoptotic proteins and HIF-1/NF-B pathway proteins was detected using Western blot assay.Results Celery extract significantly inhibited the proliferation of A498 cells,and the inhibitory effect was concentration dependent(P＜0.001).Compared with the control group,the apoptosis rates of A498 cells in the 10,20,and 40 μmol/L apigenin groups and YC-1 groups were significantly increased[(4.35±1.04)％vs(10.06±1.13)％,(18.52±2.58)％,(32.17±2.63)％,(26.94±2.41)％],as well as the expression levels of B lymphocyte tumor 2 related protein(Bax)and Cleaved Caspase-3 protein,while the expression levels of B lymphocyte tumor 2(Bcl-2)were significantly reduced(all P＜0.001).Compared with the control group,the HIF-1α protein expression levels(0.85±0.08 vs 0.63±0.06,0.31±0.03,0.16±0.02)and p-NF-KB p65/NF-KB p65 ratio(0.82±0.08 vs 0.51±0.05,0.30±0.03,0.13±0.01)of A498 cells in the 10,20,and 40 μmol/L apigenin groups were significantly reduced(all P＜0.001).Compared with the apigenin group,the apoptosis rate of A498 cells in the apigenin+DMOG group was significantly reduced[(32.17±2.63)％vs(14.85±1.62)％],and the expression levels of Bax and Cleared Caspase-3 proteins were significantly reduced,while the expression levels of Bcl-2 proteins were significantly increased(all P＜0.001).Conclusions Apigenin may promote apoptosis in renal cancer A498 cells by inhibiting the activation of the HIF-1α/NF-κB signaling pathway.
Kidney neoplasmsCell line,tumorApigeninApoptosisHypoxia-inducible factor 1,alpha subunitNF-kappa B
维普
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