The protective effect and mechanism of tea polyphenols on oral cancer in mice
Objective To explore the protective mechanism of tea polyphenols(TP)on mouse oral cancer.Methods A total of 50 mice were divided into control group,model group,TP group,Selisistat group,TP+Selisistat group,with 10 mice in each group.The control group was gavaged with physiological saline,while the model group,TP group,Selisistat group,and TP+Selisistat group were gavaged with 300 mg/L 4-NQO to establish a mouse oral cancer model.Physiological saline,200 mg/kg TP,0.01 mg/kg Selisistat,and 200 mg/kg TP+0.01 mg/kg Selisistat were gavaged respectively.The weight changes of each group of mice were compared;HE staining was used to observe the morphology of mouse oral tumor tissue;Enzyme linked immunosorbent assay was used to detect the levels of malondialdehyde(MDA)and superoxide dismutase(SOD)in serum;Immunoblotting and immunohistochemistry were used to detect the expression of silencing information regulatory factor(Sirt1)and nuclear factor E2 related factor 2(Nrf2)proteins in mouse oral tissues.Results Compared with the control group,the model group mice had a decrease in body weight[(23.19±1.36)g],a decrease in serum SOD level[(91.64±8.75)U/ml],an increase in MDA level[(5.18±0.46)nmol/ml],a decrease in Sirt1(0.38±0.05)and Nrf2(0.36±0.05)protein expression in oral tissue,and an increase in Nrf2 acetylation level(0.84±0.11)(all P＜0.05).Compared with the model group,the TP group mice had an increase in body weight[(25.28±1.25)g],elevated serum SOD levels[(121.24±10.68)U/ml],decreased MDA levels[(3.89±0.42)nmol/ml],increased expression of Sirt1(0.61±0.09)and Nrf2(0.58±0.06)proteins in oral tissue,and decreased Nrf2 protein acetylation levels(0.39±0.05);The Selisistat group mice showed a decrease in body weight[(21.41±1.07)g],a decrease in serum SOD levels[(72.16±7.43)U/ml],an increase in MDA levels[(5.87±0.41)nmol/ml],a decrease in Sirt1(0.23±0.04)and Nrf2 protein(0.24±0.03)expression in oral tissue,and an increase in Nrf2 acetylation levels(1.12±0.14)(P＜0.05).The body weight[(23.32±1.27)g],serum SOD levels[(92.58±8.13)U/ml],and oral Sirt1(0.41±0.06)and Nrf2(0.38±0.05)protein expression in the TP+Selisistat group mice were higher than those in the Selisistat group,while MDA[(5.11±0.38)nmol/ml]and Nrf2 acetylation levels(0.82±0.09)were lower than those in the Selisistat group(all P＜0.05).Conclusions Tea polyphenols can alleviate oral tissue damage and alleviate oxidative stress in mice with oral cancer,and their mechanism may be related to the upregulation of the Sirt1/Nrf2 pathway.
万方数据
维普
