The relationship between positive asthma prediction index and polymorphisms of IL-13,IL-4,ADRB2,and FcER1B genes in asthmatic children
Objective To investigate the relationship between positive asthma prediction index(API)and single nucleotide polymorphisms(SNPs)of interleukin(IL-13),IL-4,β 2 adrenergic receptor(ADRB2),and type Ⅰ Fc ε receptor β(FcER1B)genes in asthmatic children.Methods A prospective study was conducted on 102 asthmatic children under 5 years old admitted to Zhongshan Boai Hospital and Foshan First People's Hospital(51 cases were API positive and 51 cases were API negative)from January 2020 to August 2023.Oral and buccal mucosal exfoliated cells were collected from the children,and genomic DNA was extracted using magnetic bead method.Four gene loci(IL-13 rs20541,IL-4 rs2243250,ADRB2 rs1042713,FcER1B rs569108)were genotyped using a matrix assisted laser desorption ionization time-of-flight mass spectrometer.Logistic regression analysis was used to evaluate the correlation between SNP typing at these four gene loci and API positivity in asthmatic children.Results There was a statistically significant difference in the SNP typing and allele distribution frequency of IL-13 rs20541,IL-4 rs2243250,ADRB2 rs1042713,FcER1B rs569108 between the API positive and API negative groups of wheezing children(all P＜0.05).Among API positive children,the proportion of IL-13 rs20541 site was higher in GG type,the proportion of IL-4 rs2243250 site was higher in TT type,the proportion of ADRB2 rs1042713 site was higher in AG type,and the proportion of FcER1B rs569108 site was higher in AA type;The results of logistic regression analysis showed that IL-13 rs20541 GG type,IL-4 rs2243250 TT type,FcER1B rs569108 AA type were associated with the risk of API positivity in asthmatic children(all P＜0.05).Conclusions IL-13,IL-4,and FcER1B genes are risk genes for the development of API positive wheezing in children under 5 years old.SNP typing of these genes can be used to evaluate the risk of API positivity in clinical practice.
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