Expression of miRNA-144 in peripheral blood of patients with type 2 diabetes foot ulcer and its correlation with pathogenesis
Objective To investigate the expression of miRNA-144 in the peripheral blood of patients with type 2 diabetes foot ulcer(DFU)and its correlation with the pathogenesis of the disease.Methods A total of 106 patients with DFU admitted to the General Hospital of the Western Theater Command of the Chinese People's Liberation Army from March 2020 to June 2022 were retrospectively selected as the observation group,and 106 patients with type 2 diabetes mellitus(T2DM)who did not have DFU admitted to our hospital during the same period were selected as the control group.According to the median expression level of miRNA-144 in peripheral blood of DFU patients as the segmentation point,106 patients in the observation group were divided into low expression group and high expression group.We compared the clinical data of the observation group and the control group,and analyzed the relationship between different levels of miRNA-144 expression and clinical characteristics of DFU patients;A multivariate analysis was conducted on the factors related to the occurrence and development of the disease course in DFU patients,and a column chart model was constructed for model validation.Results The course of diabetes,fasting blood glucose(FPG),erythrocyte sedimentation rate(ESR),glycosylated hemoglobin(HbA1c),white blood cell count(WBC),C-reactive protein(CRP),corticotropin releasing hormone(CRH),interleukin-6(IL-6),adrenaline(E),norepinephrine(NE),cortisol(Cor)and miRNA-144 expression levels in the observation group were significantly higher than those in the control group,while transcutaneous partial pressure of oxygen(TcPO2),ankle brachial index(ABI),triglyceride(TG)and hemoglobin(Hb)were significantly lower than those in the control group,the difference was statistically significant(all P＜0.05).There were statistically significant differences in foot ulcer healing rate,Wagner grading,and ulcer course between the high expression group of miRNA-144 and the low expression group of DFU patients after 8 weeks(all P＜0.05).Multivariate regression analysis showed that the duration of diabetes＞5 years,HbA1c＞8.5％,TcPO2＜60 mmHg,CRP＞10 mg/L,Cor＞190 μg/L,and miRNA-144 expression level＞35 were independent risk factors for the occurrence of DFU in T2DM patients.The total score of 315 points after constructing the nomogram prediction model for the above factors,and the corresponding probability of occurrence and development of DFU was 72.56％.Conclusions The expression level of miRNA-144 in the peripheral blood of DFU patients is significantly related to the occurrence and development of the disease course,and the course of diabetes and the changes of HbA1c,TcPO2,CRP,Cor levels are independent risk factors for the occurrence and development of DFU,which should be focused on clinically.
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