Objective To analyze the expression levels and clinical significance of interferon(IFN)-α/β in the renal cortex and serum of children with lupus nephritis(LN).Methods A total of 32 children with LN diagnosed in the pediatric nephrology department of the Second Xiangya Hospital of Central South University from December 2017 to September 2020 were selected as the study subjects(LN group).The normal kidney control group consisted of 3 normal kidney transplant volunteers who underwent biopsy of kidney tissue(normal kidney control group),while 14 healthy children who underwent physical examination were collected as the normal control group.According to the Systemic Lupus Erythematosus Disease Activity Index(SLEDAI),LN patients were divided into mild activity group(n=8),moderate activity group(n=9),and severe activity group(n=15).According to the International Society of Nephrology/Society of Nephrology(ISN/RPS)2003 LN classification criteria,pathological classification was performed(3 cases in the mild pathological damage group,8 cases in the moderate pathological damage group,and 11 cases in the severe pathological damage group);Immunohistochemistry was used to detect the expression and distribution of IFN-α/β in glomeruli and renal interstitium;Enzyme linked immunosorbent assay(ELISA)was used to detect the concentration of IFN-α/β in serum samples and analyze its correlation with the pathological classification and disease activity of LN patients.Results The serum and renal cortex IFN-α/βlevels in the LN group were higher than those in the normal control group and normal kidney control group,respectively(all P＜0.05).The average level of serum IFN-α/β in the heavy activity group was higher than that in the light and moderate activity groups(all P＜0.05).The serum and renal cortex IFN-α/βlevels in the severe pathological damage group were significantly higher than those in the mild and moderate pathological damage groups(all P＜0.05).Conclusions IFN-α/β in the renal cortex is closely related to renal injury in LN;Serum IFN-α/β can assist in evaluating the disease activity level of LN to a certain extent.
维普
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