Studies on azaphilones isolated from the fungus Penicillium sp.YNJ-22 derived from wild poisonous mushroom and their bioactivities
The secondary metabolites,isolated from the fungus Penicillium sp.YNJ-22 derived from wild poisonous mushroom Amanita exitialis,and their bioactivities were studied.Ten azaphilones were isolated by multiple separation techniques,and the isolated compounds were identified by spectral data(mass spectrometry,nuclear magnetic resonance)as(11E)-isochromophilone Ⅳ(1),(11Z)-isochromophilone Ⅳ(2),epi-isochromophilone Ⅲ(3),sclerotioramine(4),penicilphilone A(5),geumsanol F(6),WB(7),geumsanol G(8),penicilazaphilone B(9)and geumsanol D(10).The cytotoxic activities were assayed by MTT method and the antibacterial activities were assayed by broth microdilution method.The cytotoxic activities assay showed that compound 1 displayed stronger cytotoxic activities against human lung cancer cells(A549),human breast cancer cells(MCF-7)and mouse microglia cells(BV2)[IC50=(27.37±0.13),(22.35±9.55),(26.92±1.00)μmol·L-1].Compound 4 displayed some cytotoxic activities against A549 and MCF-7 cells[IC50=(30.74±2.02),(34.58±2.45)pmnol L-1].The antibacterial activities test showed that compounds 2(MIC=128 μg·mL-1)and 3(MIC=64 μg·mL-1)exhibited some inhibitory effects on Staphylococcus aureus.The secondary metabolites of the epiphytic fungi derived from Amanita exitialis were reported for the first time,and the cytotoxic activity of compound 1 and the antibacterial activity of compound 2 were also reported for the first time.
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