首页|颅痛宁颗粒对眶下神经缩窄环术诱导三叉神经痛大鼠的治疗作用研究

颅痛宁颗粒对眶下神经缩窄环术诱导三叉神经痛大鼠的治疗作用研究

Effect of Lutongning granulethe to trigeminal neuralgia induced by chronic constriction injury of the infraorbital nerve in rats

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目的 研究颅痛宁颗粒对三叉神经痛模型大鼠的治疗作用,为其临床应用提供参考资料.方法 采用眶下神经缩窄环术(ION-CCI)制备大鼠三叉神经痛模型,大鼠随机分为正常组、假手术组、模型组、卡马西平阳性药组、颅痛宁高剂量组(2.70 g生药·kg-1·d-1)、颅痛宁低剂量组(1.35 g生药·kg-1·d-1).Von Frey毛刷测定大鼠触须垫机械痛阈值,生化法检测血液血流变和凝血功能,HE染色观察眶下神经病理变化,Western blot技术检测三叉神经内P38和 P-P38的蛋白水平.结果 颅痛宁颗粒能提高模型大鼠的痛阈值(P＜0.05,P＜0.01)降低模型大鼠血浆黏度(P＜0.05,P＜0.01)和全血还原黏度(P＜0.05,P＜0.01),提高模型大鼠血流变能力;提高模型大鼠血液凝血酶原时间(prothrombintime,PT)、活化部分凝血活酶时间(activated partial thromboplatin time,APTT)和凝血酶时间(thrombin time,TT)水平(P＜0.05,P＜0.01),降低模型大鼠纤维蛋白原(fibrinogen,FIB)水平(P＜0.01),改善凝血功能;改善模型大鼠眶下神经的病理改变;并降低模型大鼠三叉神经内 P-P38的表达量(P＜0.01).结论 颅痛宁颗粒具有改善三叉神经痛作用,可能与其活血化瘀功能有关.
Objective To investigate the therapeutic effect of Lutongning granules to rat with Trigeminal neuralgia,and provide reference materials for the clinical applications.Methods Trigeminal neuralgia was made by chronic constriction injury of infraorbital nerve in rats.Rats were randomly divided into normal group,sham group,model group,carbamazepine group,Lutongning high dose group(2.70 g crude drug·kg-1·d-1)and Lutongning low dose group(1.35 g crude drug·kg-1·d-1).Von Frey Brush brush was used to detect the mechanical pain threshold of rat whisker pad,biochemical method was used to detect the coagulation and hemostream function,HE staining was used to observe the microcirculation change of the infraorbital nerve after perfusion,western blot was used to detect the p38 and p-p38 expression in the trigeminal nerve.Results Lutongning granulethe increased the pain threshold in model rats(P<0.05,P<0.01),reduced the plasma viscosity(P<0.05,P<0.01)and the whole blood reduction viscosity in model rats(P<0.05,P<0.01),improved the flow ability in model rats,increased PT,APTT and TT levels in model rats(P<0.05,P<0.01),reduced FIB levels(P<0.01),improved the microcirculation on the infraorbital nerve in the model rats,reduced the p-p38 expression in the trigeminal nerve of model rats(P<0.01).Conclusion Lutongning granules have the effect of improving trigeminal neuralgia,which may be related to the function of promoting blood circulation and removing blood stasis.

trigeminal neuralgiachronic constriction injury of the infraorbital nerveLutongning granulethepromoting blood circulation and removing blood stasistrigeminal neuralgiaratcarbamazepine

李舒冉、郭姗姗、高双荣、包蕾、耿子涵、赵荣华、张敬升、庞博、张宇、王雅欣、徐英利、曹姗、韩冰、崔晓兰、孙静

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中国中医科学院中药研究所,北京 100029

黑龙江省济仁药业有限公司,黑龙江哈尔滨,150025

三叉神经痛 眶下神经缩窄环术 颅痛宁颗粒 活血化瘀 三叉神经痛模型 大鼠 卡马西平

国家自然科学基金中央级公益性科研院所基本科研业务费专项中国中医科学院科技创新项目

82304877ZZ15-YQ-035CI2021B015

2024

10.19803/j.1672-8629.20230591

中国药物警戒
国家药品监督管理局药品评价中心（国家药品不良反应监测中心）

中国药物警戒

CSTPCD
影响因子：1.105
ISSN：1672-8629
年,卷(期)：2024.21(3)
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