Objective To investigate the research routes of hygroscopicity of chemical reference substances.Methods Dynamic vapor absorption analysis(DVS)was mainly used to study related hygroscopicity.During the analysis with DVS,the temperature was 25℃,the gas flow rate of N2 was 200 mL·min-1,and the relative humidity changes and time were program-controlled.Results Based on a case study of pyridostigmine bromide,the three-step research routes of hygroscopicity of chemical reference substances were analyzed in depth.Conclusion Studies on related research routes can contribute to research on hygroscopicity of chemical reference substances based on water adsorption.
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