Objective To explore the molecular mechanism of Recurrent spontaneous abortion(RSA)treated by Yangxue Antai prescription.Methods RSA mouse model with kidney deficiency and blood stasis was constructed,and the placentas of mice were collected after the intervention of Yangxue Antai prescription,The pathological changes of mouse placenta were observed by HE staining and Masson staining,and the expressions of PCNA,Fibrinogen and CD34 protein in mouse placenta were determined by immunohistochemistry.The protein expression of VEGF,p-VEGFR2,p62,Beclin1,LC3Ⅰand LC3Ⅱ in mouse placenta were determined by Western blot.The ultrastructure of mouse placenta was observed by transmission electron microscopy(TEM).Results Compared with RSA group,the body weight of mice in RSA+ Yangxue Antai group increased significantly,and the embryo absorption rate decreased significantly.HE staining,Masson staining and the expression of PCNA and Fibrinogen protein showed significantly improve in mice placenta.TEM results and the protein expression of p62,Beclin1,LC3Ⅱ/LC3Ⅰ showed that the level of autophagy of the placenta of the mice was significantly decreased.CD34,VEGFA and p-VEGFR2 proteins expression showed a significant increase in placental angiogenesis.Conclusion Yangxue Antai prescription improve placental development and promote placental angiogenesis in RSA mice,which may be related to inhibiting the fusion of autophagosome and lysosome,thus inhibiting autophagy.
Yangxue Antai prescriptionrecurrent spontaneous abortionangiogenesisautophagykidney deficiency and blood stasisherbal compoundingmiceplacenta
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